Cystoviridae are a unique family of non-tailed phages with a tri-segmented dsRNA genome and a lipid envelope. They appear to be evolutionarily connected to Reoviridae, eukaryotic viruses that include pathogenic human rotaviruses. Despite intense research on the structure and functions of cystoviruses, many aspects of the phage replication cycle and its interactions with the host remain poorly understood. We use a combination of biochemical and high-throughput methods to decipher how cystoviruses regulate their protein output on the post-transcriptional level. We are further investigating which host proteins are involved in cystovirus replication and how the host responds to infection. This project will broaden our understanding of how cystoviruses take over their host to turn it into an efficient factory for replication.