SPP 2330

GROUP Wendling / WE 5822/3-1

Drivers and Constraints of Prophage Persistence in Natural Communities

Bacteriophage-antibiotic combinational treatment is a promising strategy to counter the surge of non-treatable antimicrobial resistant pathogens, and it has been successfully employed in a few critical cases. However, rational design of phage-antibiotic combinations is hindered by the limited knowledge of their underlying mechanisms. The cellular impact of antibiotics and phages alone is highly complex, and often not well understood. This is even more so for phage-antibiotic combinations, which are largely uncharacterized, and might involve molecular mechanisms beyond the regular drug and phage targeted processes. This project deploys an innovative systems biology approach to uncover the molecular underpinnings of phage-antibiotic interactions, and its interplay with the novel phage defense system CBASS.

Figure 1:Possible evolutionary drivers that can explain prophage persistence over time: (A) Prophage-bacteria combinations with high natural compatibility, for instance, due to positive epistatic interactions will stably co-exist over time. (B) Prophage-bacteria combinations with low compatibility can ameliorate prophage-imposed fitness costs via compensatory adaptations.

Principal Investigator(s)

Prof. Dr. Carolin Wendling
Ludwig-Maximilians-University Munich
Max von Pettenkofer-Institute

E-Mail: wendling@mvp.lmu.de
Homepage: https://www.mvp.uni-muenchen.de/microbiology/wendling/

PhD student(s)

Karina Krammer, krammer@mvpi.lmu.de

Publications

  • Bailey, ZM., Igler, C., Wendling, CC. (2024) Prophage maintenance is determined by environment-dependent selective sweeps rather than mutational availability” Current Biology https://doi.org/10.1016/j.cub.2024.03.025
  • Kupczok, A., Bailey, ZM., Refardt, D., Wendling, CC. (2022) “Co-transfer of functionally interdependent genes contributes to genome mosaicism in lambdoid phages” Microbial Genomics 8(11). https://doi.org/10.1099/mgen.0.000915